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AEROBIC AND ANAEROBIC BIODEGRADATION OF POLLUTANTS UNDER THE
ACTION OF BACTERIA ISOLATED FROM THE BLACK SEA WATER (I)


Maria Mirza 1), Colette Gancevici 2), Dorina Tatu-Chitoiu 2),
M. Negut 2), L. Radu 3), D. Antonescu 3), Florica Zisu 4),
Doina Caravaniez 5), Eliza Leonte 6)

1) Romanian Marine Research Institute Constanta
2) Microbiology Institute "I. Cantacuzino" Bucharest
3) National Institute of Veterinary Science "Pasteur" Bucharest
4) Transport Research Institute (INCERTRANS) Constanta
5) Research Institute for Pesticides Bucharest
6) Research and Design Institute for Wastewater Treatment Bucharest


Abstract
In the sea water mixed with industrial waste on the Black Sea coast,
in the area of Constanta, we have isolated and identified some 
aerobic bacteria: Escherichia coli, Klebsiella oxytoca, Enterobacter 
agglomerans, Proteus vulgaris, Acinetobacter calcoaceticus, Klebsiella 
pneumoniae, Pseudomonas putida, Pseudomonas piocyanea and the aerobic
germ Clostridium perfringens. We have tested the ability of those 
bacteria for using, in two mineral culture media, different concen-
trations of dissolved organic compounds: resorcinol (1,3 dihydro-
xybenzene), orcinol (3,5 hydroxytoluene), atrazine (2-choro-4-
ethylamino-6-izopropilamino-s-triazine); prometryne (2-methylthyo-4,
6-bis(isopropilamino)s-triazine); Black Sea oil; bilge and Ecomar
(an anionic oil spill dispersant sulpho-fatty acids ester). The
experiments have been conducted under laboratory conditions. We have
identified some main final products of the biodegradation by means
of infrared and visible-ultraviolet spectroscopy and thin layer
chromatography. Those experiments have allowed estimates of
biodegradation capacity of each bacterium. Optimum concentration
of tested substances and inhibition range for the tested bacterium
activities, under given experimental conditions, have been 
established.